elabscience cat e el m0676 Search Results


mouse hmgb1 elisa kit  (Elabscience Biotechnology)
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Elabscience Biotechnology mouse hmgb1 elisa kit
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Mouse Hmgb1 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits  (Boster Bio)
96
Boster Bio elisa kits
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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commercial elisa kits cat. #900-t54  (PeproTech)
90
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Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Commercial Elisa Kits Cat. #900 T54, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse?hmgb-1 (high?mobility?group?protein?b1)?elisa?kit  (Guangzhou JET Bio-Filtration)
95
Guangzhou JET Bio-Filtration mouse?hmgb-1 (high?mobility?group?protein?b1)?elisa?kit
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Mouse?Hmgb 1 (High?Mobility?Group?Protein?B1)?Elisa?Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ll/2  (ATCC)
99
ATCC ll/2
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Ll/2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12  (ATCC)
99
ATCC c2c12
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
C2c12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e el m0676 mouse il 1 beta il1b elisa kit boster cat  (Boster Bio)
94
Boster Bio e el m0676 mouse il 1 beta il1b elisa kit boster cat
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
E El M0676 Mouse Il 1 Beta Il1b Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse ifn beta ifnb1 elisa kit boster cat  (Boster Bio)
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Boster Bio mouse ifn beta ifnb1 elisa kit boster cat
Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Mouse Ifn Beta Ifnb1 Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse ifn gamma ifng elisa kit boster cat  (Boster Bio)
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Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Mouse Ifn Gamma Ifng Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse tnf alpha tnfa elisa kit boster cat  (Boster Bio)
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Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of <t>HMGB1</t> release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.
Mouse Tnf Alpha Tnfa Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of HMGB1 release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Mitoxantrone-Encapsulated ZIF-8 Enhances Chemo-Immunotherapy via Amplified Immunogenic Cell Death.

doi: 10.1002/advs.202501542

Figure Lengend Snippet: Figure 2. In vitro assessment of cellular drug uptake, tumor cytotoxic effect, and pyroptosis-boosted ICD by MIT@ZIF-8. a) CLSM images showing the intracellular distribution of MIT in CT26 cells treated with free MIT, and MIT@ZIF-8 nanoparticles for 3 hours. Scale bar: 100 μm. b) Histogram of flow cytometry analysis of MIT uptake by CT26 cells. Quantification of MIT uptake by c) CT26 cells and d) RM-1 cells using flow cytometry. Cell viability of e) RM-1 and f) CT26 cells 24 hours post-treatment with different concentrations of ZIF-8. Cell viability of g) RM-1 and h) CT26 cells after 24-hour incubation with varying concentrations of MIT or MIT@ZIF-8. i) Schematic illustration depicting ROS generation, pyroptosis induction, and enhancement of ICD by MIT@ZIF-8. j) Histogram of flow cytometry analysis of ROS levels in RM-1 cells. Quantification of ROS generation in k) RM-1 cells and l) CT26 cells by flow cytometry. m) Photographs showing morphology of RM-1 cells and CT26 cells with various treatments. Red arrows indicate cell swelling with large bubbles. Scale bar: 50 μm. n) Western blot analysis of activated caspase-1 and GSDMD levels in RM-1 and CT26 cells. 1: control, 2: MIT, 3: ZIF-8, and 4: MIT@ZIF-8. Quantification of CRT expression on the surface of o) RM-1 cells and p) CT26 cells by flow cytometry. Quantification of HMGB1 release in q) RM-1 cells and r) CT26 cells by ELISA. Data (c, d, e–h, k, l, and o–r) are expressed as mean ± s.e.m., n = 3. One-way ANOVA (c, d, g, h, k, l, and o–r) was performed. **p < 0.01, ***p < 0.001.

Article Snippet: Mouse HMGB1 ELISA kit (Cat# E-EL-M0676c) was purchased from Elabscience Bionovation Inc (China).

Techniques: In Vitro, Cytometry, Incubation, Western Blot, Control, Expressing, Enzyme-linked Immunosorbent Assay

Figure 4. In vivo anti-tumor activity and ICD induction of MIT@ZIF-8 in RM-1 tumor-bearing mice model. a) Schematic illustration for evaluating therapeutic efficacy in RM-1 tumor-bearing C57BL/6J mice. b) RM-1 tumor growth curves after treatment with MIT@ZIF-8 or 5% glucose solution (control). c) Individual tumor volume progression in MIT@ZIF-8 and control groups. d) Survival analysis of C57BL/6J mice bearing RM-1 tumors with different treatments. IHC scores of e) CRT expression on the cell surface and f) HMGB1 expression in the cell nucleus of RM-1 tumor tissues after treatment with MIT@ZIF-8 or 5% glucose solution (control). g) Representative IHC images showing CRT and HMGB1 expression in RM-1 tumor tissues upon different treatments. Scale bar: 50 μm. Data (b, e, and f) are mean ± s.e.m. Statistical analyses include two-way ANOVA (b), log-rank test (d), and Student’s t-tests (e and f). *p < 0.05, ***p < 0.001.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Mitoxantrone-Encapsulated ZIF-8 Enhances Chemo-Immunotherapy via Amplified Immunogenic Cell Death.

doi: 10.1002/advs.202501542

Figure Lengend Snippet: Figure 4. In vivo anti-tumor activity and ICD induction of MIT@ZIF-8 in RM-1 tumor-bearing mice model. a) Schematic illustration for evaluating therapeutic efficacy in RM-1 tumor-bearing C57BL/6J mice. b) RM-1 tumor growth curves after treatment with MIT@ZIF-8 or 5% glucose solution (control). c) Individual tumor volume progression in MIT@ZIF-8 and control groups. d) Survival analysis of C57BL/6J mice bearing RM-1 tumors with different treatments. IHC scores of e) CRT expression on the cell surface and f) HMGB1 expression in the cell nucleus of RM-1 tumor tissues after treatment with MIT@ZIF-8 or 5% glucose solution (control). g) Representative IHC images showing CRT and HMGB1 expression in RM-1 tumor tissues upon different treatments. Scale bar: 50 μm. Data (b, e, and f) are mean ± s.e.m. Statistical analyses include two-way ANOVA (b), log-rank test (d), and Student’s t-tests (e and f). *p < 0.05, ***p < 0.001.

Article Snippet: Mouse HMGB1 ELISA kit (Cat# E-EL-M0676c) was purchased from Elabscience Bionovation Inc (China).

Techniques: In Vivo, Activity Assay, Control, Expressing